Molecular Detection of Campylobacter and Fecal Indicators during the Northern 1 Migration of Sandhill Cranes
نویسندگان
چکیده
8 The annual Sandhill crane (Grus canadensis) migration through Nebraska, which is 9 thought to be a major source of fecal pollution to the central Platte River, is of unknown 10 human health risk. To better understand potential risks, the presence of Campylobacter 11 species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli 12 and Bacteroidetes) were assayed by PCR from crane excreta and water samples collected 13 during their stopover at the Platte River, Nebraska in 2010. Genus-specific PCR assays 14 and sequence analyses identified C. jejuni as the predominant Campylobacter species in 15 Sandhill crane excreta. Campylobacter spp. were detected in 48% of crane excreta, 24% 16 of water samples and 11% of sediment samples. Estimated densities of Enterococcus spp. 17 were highest in excreta samples (mean 4.6×10 cell equivalent (CE)/g), while water 18 samples contained higher levels of Bacteroidetes (mean 5.1×10 CE/100 mL). 19 Enterococcus spp., E. coli and Campylobacter spp. were significantly increased in river 20 water and sediments during the crane migration period, with Enterococcus spp. densities 21 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.03990-12 AEM Accepts, published online ahead of print on 12 April 2013 on July 9, 2017 by gest ht://aem .sm .rg/ D ow nladed fom
منابع مشابه
Molecular detection of Campylobacter spp. and fecal indicator bacteria during the northern migration of sandhill cranes (Grus canadensis) at the central Platte River.
The risk to human health of the annual sandhill crane (Grus canadensis) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli, and Bacteroidetes) was assayed by PCR from...
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